作者：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.
摘要：The marker-free transgenic Citrus was investigated by combining application of a chemical regulated induction system XVE with, Cre/loxP-mediated site-specific DNA recombination in a single transformation to remove carriers. the system can remove marker gene (nptⅡ) by expression of Cre recombinase system, which was tightly controlled by a system upon induction by β-estradiol. The infected internodal stem segments of "succari" sweet orange as explants were cultured on co-cultivation medium (MS+3 mg/LBA), After 3 d, they were transformed onto selection medium (MS+75mg/L Kan+500 mg/L Cef). After selection, regeneration buds grow, with 1-2mm explants were cut and transferred onto chemical-inducible medium (MS+3mg/L BA+500mg/L Cef +2μmol/L β-estradiol). After 15 d of continuous culture, the express of gfp could be observed successfully by fluorescence micoscope. Finally,Molecular detection ibdicated that the DNA recombination and excision in transgenic Citrus had already occurred after 30 d grafted in vitro. The feasibility that this chemical-inducible excising marker gene systen could be applied onto Citrus was proved and therefore, an efficient marker-free transgenic system in Citrus could be estanblished.
关键词：Citrus; marker-free transgenic system; chemical-inducible; Marker gene; β-estrad
Key words: 柑橘;无标记转基因体系;化学诱导;标记基因;β-雌二醇
文献注录：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.. 柑橘无标记转基因转化体系的建立 [J]. 湖南农业大学学报(自然科学版). 2010, 06: 649-652.
报/刊名：《湖南农业大学学报(自然科学版)》，发表于2010 / 第 06 期。
文献类型： [J]
页码：第 649-652 页 / 共4 页