作者：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.
摘要：The marker-free transgenic Citrus was investigated by combining application of a chemical regulated induction system XVE with,Cre/loxP-mediated site-specific DNA recombination in a single transformation to remove carriers.The system can remove marker gene(nptⅡ)by expression of Cre recombinase system,which was tightly controlled by a system upon induction byβ-estradiol.The infected internodal stem segments of‘Succari’sweet orange as explants were cultured on co-cultivation medium(MS+3 mg/L BA),After 3 d,they were transformed onto selection medium(MS+75 mg/L Kan+500 mg/L Cef).After selection,regeneration buds grow,with 1~2 mm explants were cut and transferred onto chemical-inducible medium (MS+3 mg/L BA+500 mg/L Cef+2μmol/Lβ-estradiol).After 15 d of continuous culture,the express of gfp could be observed successfully by fluorescence microscope. Finally,Molecular detection indicated that the DNA recombination and excision in transgenic Citrus had already occurred after 30 d grafted in vitro.The feasibility that this chemical-inducible excising marker gene system could be applied onto Citrus was proved and therefore,an efficient marker-free transgenic system in Citrus could be established.
关键词：Citrus;marker-free transgenic system;chemical-inducible;marker gene;β-estradiol
Key words: 柑橘;无标记转基因体系;化学诱导;标记基因;β-雌二醇
文献注录：李琳洁, 杨莉, 李芳, 谢玉明, 曾柏全, 邓子牛.. 柑橘无标记转基因转化体系的建立 [J]. 湖南农业大学学报(自然科学版). 2010, 649-6.
报/刊名：《湖南农业大学学报(自然科学版)》，发表于2010 / 第 649-6 期。
文献类型： [J]
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