作者：罗赛男, 邓子牛, 钟晓红, 张家银, 袁飞荣, 杨莉.
摘要：A parthenocarpy gene(DefH9-iaaM)was introduced into Succari to obtain possible seedless transformants, by using internodal stem segments as explants to establish an efficient regeneration and transgenic system.The results indicated that 30-day-old seedlings were the most efficient explants.The co-culture in darkness for 3~6 days gave a rise to good regeneration.The optimum medium for adventitious buds regeneration was MS+6-BA 5.0 mg/L.And MS medium supplied with 6-BA1.0 mg/L and NAA0.1 mg/L showed the highest bud multiplication rate，3.3 buds on each internodal segments.The shoots successfully rooted after 20 days in 0.5 mg/L NAA.After Agrobacterium-mediated transformation，6 transgenic plants out of 17 regenerants were identified by PCR.
关键词：succari sweet orange；regeneration system；transformation；DefH9-iaaM gene；partheno
Key words: 糖橙;再生体系;转基因;DefH9-iaaM基因;单性结实
文献注录：罗赛男, 邓子牛, 钟晓红, 张家银, 袁飞荣, 杨莉.. 用单性结实基因defH9-iaaM转化糖橙的研究 [J]. 湖南农业大学学报(自然科学版). 2008, 02: 177-181.
报/刊名：《湖南农业大学学报(自然科学版)》，发表于2008 / 第 02 期。
文献类型： [J]
页码：第 177-181 页 / 共5 页