作者：王会全, 曾黎辉, 吴少华, 徐海峰.
摘要：To inoculate A．tumefaciens into the embryonic apical meristerm of the soaked seeds，a region on the seed suiface where a shoot would later emerge was pierced twice up to a depth of about 1 mm with a needle dipped in the A．tumefaciens inoculum．Germinated seedings were transplanted．One transgenic plantlet was obtained，which was confirmed by PCR assay and PCR-Southern blot．The transformation rate reached to 4.35%，and the feasibility of this method was proved，which could provid reference for the transgene of woody trees．
关键词：Agrobacterium-mediated; Genetic transformation; Xuegan; LFY gene; Method of
Key words: 农杆菌介导;遗传转化;雪柑;LFY基因;针刺法
文献注录：王会全, 曾黎辉, 吴少华, 徐海峰.. 一种根癌农杆菌介导的简单、快捷转化雪柑的新方法 [J]. 生物技术通报. 2011, 09: 176-180.
报/刊名：《生物技术通报》，发表于2011 / 第 09 期。
文献类型： [J]
页码：第 176-180 页 / 共5 页