作者：谢玉明, 郭琛, 张家银, 韩健, 袁飞荣, 邓子牛.
摘要：The phyB gene was transferred into the genome of Poncirus trifoliate in vitro with explants of epicotyl mediated by Agrobacterium．To诅l 27 strains with plant resistance plants were regenerated．The presence of specific targeted strips of phyB gene in the 5 regenerated plants Was confirmed by PCR analysis．RT-PCR analysis indicated the regular expression of transgene phyB in two transgenic clones，phyB3 and phyB4 in omparison with the control plants，the phyB transgenic plants had higher net photosynthesis rate．All the regenerated transgenic plants，as compared with the untransformed clones，showed lower height，shorter interval between intemodes and smaller leafarea．
关键词：Agrobactedum-mediated；phyB gene；genetic transformation；Poncirus trifoliate
Key words: 农杆菌介导法;phyB基因;遗传转化;枳壳
文献注录：谢玉明, 郭琛, 张家银, 韩健, 袁飞荣, 邓子牛.. 农杆菌介导phyB基因转化枳壳研究 [J]. 湖南农业大学学报(自然科学版). 2008, 01: 68-72.
报/刊名：《湖南农业大学学报(自然科学版)》，发表于2008 / 第 01 期。
文献类型： [J]
页码：第 68-72 页 / 共5 页