作者：谢玉明, 郭琛, 张家银, 杨丽, 胡春华, 邓子牛.
摘要：Genetic transformation systems from mature tissue of citrus have to be achieved as a necessary requiremerit for the introduction of useful genes into specific cultivars and the rapid evaluation of the genetically modifled characteristic．This research presents a protoeol for in vitro regeneration and genetic transformation of C. sinensis Osbesk cv Newhall using as explants intemodal segments from adult plants maintained in the greenhouse．The contamination rate of the pretreated disinfection method was the lowest．Adventitious buds induction for C. sinensis Osbesk CV Newhall was the most effective on MS medium supplemented with 3 mg／L 6-BA．chit42 gene was introduced to the C．sinensis Osbesk cv Newhall by Agrobacterium-mediated. Adventitious buds were micro-grafted onto seedlings of Carrizo citrange grown in vitro and one month later re-grafted On greenhouse-grown rootstocks．By PCR detection, we obtained 2 transformed plants, RT—PCR analysis indicated that the transgene chit42 regularly expressed in the transgenic plant.
关键词：Csinensis Osbeck cv.; Newhall,Matrue intemodal segments,Genetic仃ansformation,chi
Key words: 纽荷尔脐橙，成年态节间茎段，遗传转化，chit42基因
文献注录：谢玉明, 郭琛, 张家银, 杨丽, 胡春华, 邓子牛.. 纽荷尔脐橙成年态节间茎段遗传转化研究 [J]. 分子植物育种. 2008, 05: 1019-1024.
报/刊名：《分子植物育种》，发表于2008 / 第 05 期。
文献类型： [J]
页码：第 1019-1024 页 / 共6 页