作者：杨莉, 徐昌杰, 陈昆松.
摘要：An effieient in vitro regeneration system and Agrobacterium-mediated transformation system were established in kumquats (Fortunella crassifolia swingle), and more than 20 transgenic plants were obtained. Three protoeols, multiplex PCR,T一DNA flanking sequence analysis and Southern blotting, were applied to early, middle and late analysis of transformation，respectively. With the established transformation system, antisense orange β一earotene hydroxylase gene was introduced into kumquat in order to produce high β一earotene fruits. Meanwhile, transgenic plants incorporating GUS gene under an orange chlorophyllase gene promoter were obtained to evaluate the expression pattern of the promoter.
关键词：kumquat(Fortunella erassifolia Swingle); regeneration;transformation; transforma
Key words: 金柑;组织培养;遗传转化;转基因检测;甜橙β-胡萝卜素经化酶反义基因;甜橙叶绿素酶启动子
文献注录：杨莉, 徐昌杰, 陈昆松.. 金柑遗传体系的建立及应用 [J]. 《中国园艺学会第七届青年学术讨论会论文集》. 2006: 555-558.
报/刊名：《《中国园艺学会第七届青年学术讨论会论文集》》，发表于2006
文献类型： [J]
页码：第 555-558 页 / 共4 页