作者：杨晓红, 孙中海, 仝瑞建.
摘要：Genetic transformation experiments of the different exploids from Citrus grandis cv.Changshou Shatian You infected with Agrobacterium rhizogenes were carried out under dark and light.The optimized culture system for Ri T-DNA transformed roots of C.grandis cv.Changshou Shatian You was constructed as follows:After the ventral wounded striations on the single activation cotyledon were inoculated by A.rhizogenes A4(logarithmic period),they were cultured at the temperature of 25±2℃in the dark for 25-30 days,some transformed roots were generated from wounded striations of most cotyledons.The genetic transformation ratio was about(83±11)%.Axenic Ri T-DNA transformed roots(hairy roots)were harvested after five subcultures. Explants were activated on MT medium.The MS medium was used for subculture of transformed roots.Mass Ri T-DNA transformed roots which the hormone was produced independently were harvested from this optimized culture system.White fresh Ri T-DNA transformed roots were about 1.14±0.07 cm long,0.73±0.04 mm wide,and grew negative geotropism.
关键词：Citrus grandis cv.Changshou Shatian You; Agrobacterium rhizogenes; Ri T-DNA tran
Key words: 长寿沙田柚;发根农杆菌;Ri;T-DNA转型根;优化培养体系
文献注录：杨晓红, 孙中海, 仝瑞建.. 长寿沙田柚Ri T-DNA转型根优化培养体系研究 [J]. 中国农业科学. 2005, 12: 2496-2501.
报/刊名：《中国农业科学》，发表于2005 / 第 12 期。
文献类型： [J]
页码：第 2496-2501 页 / 共6 页