作者：Soriano L, Mourao FDA, Camargo LEA, Cristofani-Yaly M, Latado RR, Pacheco CD, Azevedo FA, Mendes BMJ.
作者单位：Universidade de São Paulo,2Centro APTA Citros Sylvio Moreira
摘要：Protoplast fusion between sweet orange and mandarin/mandarin hybrids scion cultivars was performed following the model "diploid embryogenic callus protoplast + diploid mesophyll-derived protoplast". Protoplasts were isolated from embryogenic calli of 'Pera' and 'Westin' sweet orange cultivars (Citrus sinensis) and from young leaves of 'Fremont', Nules', and 'Thomas' mandarins (C. reticulata), and 'Nova' tangelo [C. reticulata x (C. paradisi x C. reticulata)]. The regenerated plants were characterized based on their leaf morphology (thickness), ploidy level, and simple sequence repeat (SSR) molecular markers. Plants were successfully generated only when 'Pera' sweet orange was used as the embryogenic parent. Fifteen plants were regenerated being 7 tetraploid and 8 diploid. Based on SSR molecular markers analyses all 7 tetraploid regenerated plants revealed to be allotetraploids (somatic hybrids), including 2 from the combination of 'Pera' sweet orange + 'Fremont' mandarin, 3 'Pera' sweet orange + 'Nules' mandarin, and 2 'Pera' sweet orange + 'Nova' tangelo, and all the diploid regenerated plants showed the 'Pera' sweet orange marker profile. Somatic hybrids were inoculated with Alternaria alternata and no disease symptoms were detected 96 h post-inoculation. This hybrid material has the potential to be used as a tetraploid parent in interploid crosses for citrus scion breeding.
关键词：Alternaria brown spot,Disease resistance,Protoplast fusion,Tetraploid,breeding,p
文献注录：Soriano L, Mourao FDA, Camargo LEA, Cristofani-Yaly M, Latado RR, Pacheco CD, Azevedo FA, Mendes BMJ.. Regeneration and characterization of somatic hybrids combining sweet orange and mandarin/mandarin hybrid cultivars for citrus scion improvement [J]. Plant Cell, Tissue and Organ Culture (PCTOC), . 2012, 03: 385-392.
报/刊名：《Plant Cell, Tissue and Organ Culture (PCTOC), 》，发表于2012 / 第 03 期。
文献类型： [J]
页码：第 385-392 页 / 共8 页