作者：Cai, X. D.,Fu, J.,Chen, C. L.,Guo, W. W.
作者单位：1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
摘要：Somatic hybridization provides an alternative for transferring mitochondria-encoded cytoplasmic male sterility (CMS). Herein, symmetric protoplast electrofusion was conducted between embryogenic callus protoplasts of Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a CMS cultivar, and mesophyll-derived protoplasts of seedy 'Page' tangelo [C. reticulata Blanco x (C. reticulata Blanco x C. paradisi Macf.)] or 'Nova' tangelo [C. reticulata Blanco x (C. reticulata Blanco x C. paradisi Macf.)], to transfer CMS trait. Flow cytometry analysis showed that 14 plants recovered from G1 + 'Page' tangelo that displayed typical morphological character of 'Page' were diploid, and 6 plants regenerated from G1 + 'Nova' tangelo were tetraploid. Genetic compositions of regenerated plants from the two fusions were determined by SSR, CAPS and chloroplast-SSR analysis. Cybrid nature of diploids from G1 + 'Page' tangelo with nuclear DNA from 'Page', mitochondrial DNA (mtDNA) from the G1 and chloroplast DNA (cpDNA) derived from either parent was confirmed. Tetraploid plants from G1 + 'Nova' tangelo were identified as somatic hybrids with random cpDNA inheritance. The regenerated cybrid and hybrid plants hold great potential for Citrus seedless breeding at diploid or triploid levels. (C) 2009 Elsevier B.V. All rights reserved.
关键词：Citrus,Cybrid,Seedlessness,molecular characterization,mitochondrial genomes, hy
文献注录：Cai, X. D.,Fu, J.,Chen, C. L.,Guo, W. W.. Cybrid/hybrid plants regenerated from somatic fusions between male sterile Satsuma mandarin and seedy tangelos [J]. Scientia Horticulturae. 2009, 02: 323-327.
报/刊名：《Scientia Horticulturae》，发表于2009 / 第 02 期。
文献类型： [J]
页码：第 323-327 页 / 共5 页