作者：Guo, W. W.,Cheng, Y. J.,Chen, C. L.,Deng, X. X.
作者单位：1National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China
摘要：More than 150 plants were regenerated from our previous somatic hybridization between embryogenic callus line of Page tangelo (Citrus reticulata Blanco x C. paradisi Macf) and mesophyll protoplasts of rough lemon (C. jambhiri Lush) mediated by electrofusion. Preliminary screening showed that 78% of these plants were tetraploids while the rest were diploids morphologically resembling the leaf parent (rough lemon). Herein, eight plants (six tetraploids and two diploids) were selected and further analyzed by flow cytometry, simple sequence repeat (SSR), mitochondria (mt) RFLP and chloroplast (cp) SSR techniques. The results showed that four of these six tetraploids were somatic hybrids, one tetraploid was autotetraploid of Page tangelo, and the remaining one tetraploid was cybrid with nuclear and cpDNA from rough lemon and mtDNA from Page tangelo; the two diploids were verified being cybrids with nuclear DNA from rough lemon and mtDNA from Page tangelo, cpDNA was randomly inherited. The regeneration mechanism of these novel cybrids was discussed. (c) 2006 Elsevier B.V. All rights reserved.
关键词：citrus,somatic hybridization,autotetraploid, cybrid, molecular markers, protop
文献注录：Guo, W. W.,Cheng, Y. J.,Chen, C. L.,Deng, X. X.. Molecular analysis revealed autotetraploid, diploid and tetraploid cybrid plants regenerated from an interspecific somatic fusion in Citrus [J]. Scientia Horticulturae. 2006, 02: 162-166.
报/刊名：《Scientia Horticulturae》，发表于2006 / 第 02 期。
文献类型： [J]
页码：第 162-166 页 / 共5 页