作者：Takami, K.,Matsumaru, A.,Yahata, M.,Kunitake, H.,Komatsu, H.
作者单位：1Faculty of Agriculture, University of Miyazaki,2School of Agriculture, Kyushu Tokai University
摘要：Embryogenic protoplasts of 'Shogun' mandarin ( Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from Citropsis gabunensis Swing. & M. Kell, and two green embryoids were regenerated after 3 months of culture. Two months after transfer to the regeneration medium, numerous plantlets were obtained from the embryoids. These plants grew vigorously, had well-developed root systems, and exhibited leaf characteristics intermediate to those of the parents. The absolute nuclear genome size of the regenerated plant SH2 ( 1.75 pg/4C) was the sum of those of the 'Shogun' mandarin ( 0.75 pg/2C) and C. gabunensis ( 0.97 pg/2C). The chromosome counts of the young leaves revealed that they were tetraploids ( 2n= 4x= 36). Random amplified polymorphic DNA ( RAPD) analysis of the two lines ( SH1 and SH2) verified their hybridity. Cytoplasmic genome analysis using universal primers reveal that their chloroplast ( cp) DNA banding pattern is identical to that of C. gabunensis, while the banding pattern of the mitochondrial ( mt) DNA is identical to that of the 'Shogun' mandarin. These somatic hybrids are important materials for investigating the phylogenetic relationships between these two genera in the subfamily Aurantioideae.
关键词：protoplast fusion,flow cytometry,nuclear genome size, Aurantioideae, taxonomy
文献注录：Takami, K.,Matsumaru, A.,Yahata, M.,Kunitake, H.,Komatsu, H.. Utilization of intergeneric somatic hybrids as an index discriminating taxa in the genus Citrus and its related species [J]. Sexual Plant Reproduction. 2005, 01: 21-28.
报/刊名：《Sexual Plant Reproduction》，发表于2005 / 第 01 期。
文献类型： [J]
页码：第 21-28 页 / 共8 页