作者：Cabasson, C. M.,Luro, F.,Ollitrault, P.,Grosser, J. W.
作者单位：1CIRAD-FLHOR, B.P. 5035, 34032 Montpellier, France,2Station de Recherches Agronomiques, INRA-CIRAD, San Giuliano, 20230 San Nicolao, Corsica, France,3Citrus Research and Education Center, University of Florida, IFAS, 700 Experiment Station Road, Lake
摘要：Somatic hybridization offers the possibility of manipulating chloroplast and mitochondrial genomes and evaluating their role on cultivar qualities in citrus. Numerous associations between Willow-leaf mandarin (Citrus deliciosa Ten.), as embryogenic parent, and sweet orange cv. Valencia (Citrus sinensis (L.) Osb.), as mesophyll parent, and between Willow-leaf mandarin (embryogenic parent) and grapefruit cv. Duncan (Citrus paradisi Macf.) (mesophyll parent) were obtained by the fusion of protoplasts induced by polyethylene glycol. Regenerated plants were characterized by flow cytometry and nuclear and mitochondrial DNA restriction fragment length polymorphism (RFLP). All plants were diploid. Diploid plants with the nuclear RFLP patterns of mandarin or sweet orange were identified in the progeny between these two parents, while only grapefruit nuclear types were found in the mandarin + grapefruit progeny. The diploid plants with the nuclear profile of the mesophyll parent originated systematically from cells formed through spontaneous association of the nuclear genome of the mesophyll parent and the mitochondrial genome of the embryogenic parent. These plants are assumed to be alloplasmic hybrids or cybrids. They were viable and have been propagated for field testing.
关键词：Citrus,RFLP,mitochondrial DNA,nuclear DNA,protoplast fusion,somatic hybridizatio
文献注录：Cabasson, C. M.,Luro, F.,Ollitrault, P.,Grosser, J. W.. Non-random inheritance of mitochondrial genomes in Citrus hybrids produced by protoplast fusion [J]. Plant Cell Reports. 2001, 07: 604-609.
报/刊名：《Plant Cell Reports》，发表于2001 / 第 07 期。
文献类型： [J]
页码：第 604-609 页 / 共6 页