作者：Liu, J. H.,Deng, X. X.
摘要：Protoplasts of Valencia sweet orange ( Citrus sinensis Osb.),irradiated by X-ray with a dose rate of 3.8 krad/min for 45 min, were electrically fused with protoplasts of Murcott tangor ( C. reticulata x C. sinensis) that were treated with 0.25 mmol/L iodoacetic acid for 15 min. It took nearly 15 months for the fusion-derived calli to develop into embryoids that were only originated in the medium of MT supplemented with 2% glycerol. The shoots were recalcitrant to rooting in the root-induction medium. In vitro grafting was employed to produce whole plants though one self-rooting plant was obtained. Cytological determination of root and shoot tips showed mainly diploid and aneuploid cells, together with few tetraploid cells in some plants. RAPD (random amplified polymorphism DNA) analysis with 10-mer primers demonstrated that bands specific to the fusion parents were detected in the regenerated plants, indicating that interspecific somatic hybrids have been obtained via protoplast asymmetric fusion in Citrus.
关键词：Citrus,asymmetric fusion,aneuploid cell,RAPD,somatic hybrid,lycopersicon-esculen
文献注录：Liu, J. H.,Deng, X. X.. Regeneration and characterization of plants derived from asymmetric protoplast fusion in Citrus [J]. Acta Botanica Sinica. : 1144-1149.
报/刊名：《Acta Botanica Sinica》，发表于
文献类型： [J]
页码：第 1144-1149 页 / 共 页