作者：Motomura, T.,Moriguchi, T.,Moriguchi, T.,Hidaka, T.,Omura, M.
摘要：Somatic hybrids were produced by protoplast fusion between embryogenic callus cells of 'Hazzara (Abohar)' (Citrus reticulata Blanco) and mesophyll cells of Microcitrus australis (Planch.) Swingle, in an attempt to expand genetic diversity that might be valuable for Citrus breeding. Among the 40 plants acclimatized, 37 plants were intermediate in leaf morphology, whereas, the remaining 3 had leaves quite different from those of both parents. Ten plants among those with intermediate leaf morphology were selected for analysis of genome composition. These plants proved to be somatic hybrids by nuclear rDNA analysis. Mitochondrial DNA (mtDNA) analysis showed that all 10 hybrids contained 'Hazzara (Abohar)' plastome. Analysis of chloroplast DNA (cpDNA) showed that roost of them carried specific fragments derived from 'Hazzara (Abohar)'. Others, however, carried specific fragments derived from both parents. Possible causes for such band patterns are discussed from a genetical viewpoint.
关键词：chloroplast dna distribution,protoplast-fusion,parasexual hybrids,nicotiana-taba
文献注录：Motomura, T.,Moriguchi, T.,Moriguchi, T.,Hidaka, T.,Omura, M.. Analysis of cytoplasmic genomes in somatic hybrids between 'Hazzara (Abohar)' (Citrus reticulata Blanco) and Microcitrus australis (Planch) swingle [J]. Journal of the Japanese Society for Horticultural Science. : 497-503.
报/刊名：《Journal of the Japanese Society for Horticultural Science》，发表于
文献类型： [J]
页码：第 497-503 页 / 共 页