作者：Saito, W.,Ohgawara, T.,Shimizu, J.,Ishii, S.,Kobayashi, S.
摘要：Through the cell fusion between nucellar callus cells and mesophyll cells in two Citrus combinations, we obtained the regenerated plants resembling mesophyll parent besides the somatic hybrids. In the fusion experiment between sudachi (Citrus sudachi Hort.) and lime (C. aurantifolia Swingle), 4 out of 12 regenerated clones were similar to mesophyll parent (lime) as we already reported. In another experiment of sudachi and lemon (C. limon Burn) 4 out of 6 clones were similar to mesophyll parent (lemon). These regenerated plants had 18 chromosomes, which were equal to diploid parents and showed the same nuclear rDNA fragment patterns as those of mesophyll parents. The composition of the mitochondrial genomes of these regenerated plants was investigated. The mtDNA analysis revealed that all of the clones that resembled mesophyll parents had identical mtDNA fragment patterns to those of the nucellar callus parent, indicating that these regenerated clones resembling mesophyll parents were cybrids. These results suggest that the mitochondria of nucellar cells may play a significant role in Citrus embryogenesis.
关键词：protoplast-fusion,cybrids,acid citrus,rdna,mtdna,somatic hybrid plants,protoplas
文献注录：Saito, W.,Ohgawara, T.,Shimizu, J.,Ishii, S.,Kobayashi, S.. CITRUS CYBRID REGENERATION FOLLOWING CELL-FUSION BETWEEN NUCELLAR CELLS AND MESOPHYLL-CELLS [J]. Plant Science. : 195-201.
报/刊名：《Plant Science》，发表于
文献类型： [J]
页码：第 195-201 页 / 共 页