作者：Jin, S. B,Sun, H. J,Al Bachchu, M. A,Chung, S. J,Lee, J,Han, S. I,Yun, J. H,Boo, K. W,Lee, D.Riu, K. Z.Kim, J. H.
作者单位：Subtropica/Tropical Plant Gene Bank, Jeju National University, Subtropical Horticulture Research Institute, Jeju National University,Faculty of Biotechnology, College of Applied Life Science, Jeju NationalUniversity, SARI
摘要：Miraculin gene containing the N-terminal signal peptide was introduced into navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) callus cells by Agrobacterum-mediated transformation. Transgenic somatic embryos were screened on the shoot induction medium containing 25 mg hygromycin L-1. Citrus callus cells were reproduced from the green color somatic embryos on the callus reproduction medium. The obtained transgenic cells were cultured in Murashige and Tucker's liquid medium containing 50 g sucrose in a shaking incubator. Similar to the native miraculin, the secreted recombinant miraculin protein formed a disulfide-linked dimer and retained taste-modifying activity. The stability of recombinant protein expression was confirmed over nine generations of callus. This production system can be an excellent alternative for producing various recombinant proteins as well as miraculin.
关键词：Citrus callus,Miraculin,Recombinant protei,Suspension cell culture,taste-modifyi
文献注录：Jin, S. B,Sun, H. J,Al Bachchu, M. A,Chung, S. J,Lee, J,Han, S. I,Yun, J. H,Boo, K. W,Lee, D.Riu, K. Z.Kim, J. H.. Production of Recombinant Miraculin Protein Using Transgenic Citrus Cell Suspension Culture System [J]. Journal of the Korean Society for Applied Biological Chemistry. 2013, 03: 271-274.
报/刊名：《Journal of the Korean Society for Applied Biological Chemistry》，发表于2013 / 第 03 期。
文献类型： [J]
页码：第 271-274 页 / 共4 页