作者：Zou, X. P.，Song, E. L，Peng, A. H.，He, Y. R.，Xu, L. Z.，Lei, T. G.，Yao, L. X.，Chen, S. C.
摘要：To improve the resistance of citrus to canker disease caused by Xanthomonas axonopodis pv. citri (Xac), it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated the functionality of PPP1 and hsr203J (Nicotiana tabacum L.) and gst1 (potato) pathogen-inducible promoters to drive expression of the beta-glucuronidase (GUS) reporter gene in transgenic citrus. The activities of these promoters in response to the Xac pathogen and wounding were determined quantitatively and qualitatively by fluorometric and histochemical GUS assays, and compared with that of the cauliflower mosaic virus (CaMV) 35S promoter. In citrus, the hsr203J promoter from tobacco was hardly activated by the Xac pathogen or wounding, whereas the PPP1 and gst1 promoters were rapidly and efficiently activated by both inducers. There was very little visible background expression from the PPP1 promoter, but a high level of background expression from the gst1 promoter. Because of its low background expression, the PPP1 promoter was more responsive to Xac and wounding than was the gst1 promoter. However, the gst1 promoter was more rapidly activated than the PPP1 promoter by Xac and wounding. The inducible activity of the two promoters was restricted to infection sites. Taken together, our results showed that the PPP1 promoter was the most efficient promoter among those evaluated in this study. Its strong responsiveness to Xac and wounding suggests that it would be a good candidate for expression of antibacterial transgenes specifically at infection sites to improve canker disease resistance in citrus.
关键词：Pathogen-inducible promoter,Citrus canker,Xanthomonas axonopodis pv.,citri (Xac)
文献注录：Zou, X. P.，Song, E. L，Peng, A. H.，He, Y. R.，Xu, L. Z.，Lei, T. G.，Yao, L. X.，Chen, S. C.. Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding [J]. Plant Cell Tissue and Organ Culture. 2014, 01: 85-98.
报/刊名：《Plant Cell Tissue and Organ Culture》，发表于2014 / 第 01 期。
文献类型： [J]
页码：第 85-98 页 / 共14 页