作者：Cardoso, Jean C.Cardoso, Jean C.Germana, Maria Antonieta,Latado, Rodrigo R.
作者单位：Univ Sao Paulo, CENA, BR-13416903 Piracicaba, SP, Brazil,Univ Palermo, Dipartimento Sci Agr & Forestali, I-90128 Palermo, Italy,Ctr APTA Citros Sylvio Moreira IAC, Cordeiropolis, SP, Brazil
摘要：Citrus, and particularly sweet oranges, are very recalcitrant to anther culture. In this paper it was evaluated for the first time the response of 27 genotypes of Citrus sinensis and of one hybrid C. clementina x C. sinensis, to in vitro anther culture. Ten genotypes of sweet oranges showed embryogenic callus induction, mostly blood sweet oranges genotypes, such as Tarocco, Moro and Sanguinelli. In vitro microspore developmental switches from the gamethophytic to the sporophytic pathway were shown by DAPI staining in microspores of these responsive genotypes, after 10 months in culture. However, microsatellite marker analyses showed that these calli were heterozygous. The flow-cytometric analysis of these embryogenic calli showed the presence of two peaks, corresponding to haploid (n) and diploid (2n) genotypes. Differently, anther cultures of the hybrid C. clementina x C. sinensis produced tri-haploid (3n) embryogenic calli and the embryos obtained were homozygous when analyzed by molecular markers (sample sequence repeats), confirming the more responsive characteristic of clementine to microspore embryogenesis through anther culture.
关键词：Citrus Microsporogenesis Calli Haploid ,Tri haploid Molecular profil
文献注录：Cardoso, Jean C.Cardoso, Jean C.Germana, Maria Antonieta,Latado, Rodrigo R.. In vitro anther culture of sweet orange (Citrus sinensis L. Osbeck) genotypes and of a C. clementina x C. sinensis \'Hamlin\' hybrid [J]. Plant Cell Tissue and Organ Culture. 2014, 3(117): 455-464.
基金: FAPESP ,CNPq
报/刊名：《Plant Cell Tissue and Organ Culture》，发表于2014 / 第 3(117) 期。
文献类型： [J] （文献级别：SCI刊物）
页码：第 455-464 页 / 共10 页