作者：Shi-Xiao Xu • Dong-Fang Cai • Feng-Quan Tan •Yan-Ni Fang • Kai-Dong Xie •, Jude W. Grosser ̶
作者单位：Key Laboratory of Horticultural Plant Biology (Ministry ofEducation), Huazhong Agricultural University, Wuhan,China,College of Tobacco Science, Henan Agricultural University,Zhengzhou 450002, China,Citrus Research and Education Center, University of
摘要：Citrus somatic hybrids produced in the past years provide a novel opportunity to study the immediate effects of allopolyploidization on genome structure and methylation. Here, we present a first attempt to investigate the alterations in genome structure and methylation in three sets of citrus somatic allotetraploids and their diploid parents using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) techniques. Our results indicate that all the allotetraploids mainly have the AFLP and MSAP banding patterns containing specific bands from both parents plus some alterations. The incidences of the AFLP polymorphic bands in allotetraploids show a range from 4.61 to 7.88 %, while from 12.50 to 15.67 % of the sites are methylated. In addition, the proportions of callus-parent-specific DNA structure and methylation alterations are much greater than those of leaf-parent-specific alterations in the somatic hybrids. Furthermore, we find that the somatic hybrids take on a greater divergence from the callus parent and a closer relationship to leaf parent in all groups of plants by dendrogram analysis based on AFLP or MSAP data. Taken together, our results suggest that somatic hybrids are very useful in elucidating the immediate changes that occur in newly synthesized allotetraploid.
关键词：Citrus Somatic hybrid Allotetraploid Epigenetics DNA methylation
文献注录：Shi-Xiao Xu • Dong-Fang Cai • Feng-Quan Tan •Yan-Ni Fang • Kai-Dong Xie •, Jude W. Grosser ̶. Citrus somatic hybrid: an alternative system to study rapid structural and epigenetic reorganization in allotetraploid genomes [J]. Plant Cell Tissue and Organ Culture. 2014, 3(119): 511-522.
报/刊名：《Plant Cell Tissue and Organ Culture》，发表于2014 / 第 3(119) 期。
文献类型： [J]
页码：第 511-522 页 / 共12 页