作者：Xiuping Zou • Erling Song • Aihong Peng •Yongrui He • Lanzhen Xu • Tiangang Lei •Lixiao Ya
作者单位：Citrus Research Institute, Chinese Academy of AgriculturalSciences, Chongqing 400712, People’s Republic of China,National Center for Citrus Variety Improvement,Chongqing 400712, People’s Republic of China
摘要：To improve the resistance of citrus to canker
disease caused by Xanthomonas axonopodis pv. citri (Xac),
it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated
the functionality of PPP1 and hsr203J (Nicotiana tabacum
L.) and gst1 (potato) pathogen-inducible promoters to drive
expression of the b-glucuronidase (GUS) reporter gene in
transgenic citrus. The activities of these promoters in
response to the Xac pathogen and wounding were determined quantitatively and qualitatively by fluorometric and
histochemical GUS assays, and compared with that of the
cauliflower mosaic virus (CaMV) 35S promoter. In citrus,
the hsr203J promoter from tobacco was hardly activated by
the Xac pathogen or wounding, whereas the PPP1 and gst1
promoters were rapidly and efficiently activated by both
inducers. There was very little visible background
expression from the PPP1 promoter, but a high level of
background expression from the gst1 promoter. Because of
its low background expression, the PPP1 promoter was
more responsive to Xac and wounding than was the gst1
promoter. However, the gst1 promoter was more rapidly
关键词：Pathogen-inducible promoter Citrus canker Xanthomonas axonopodis pv. citri (X
文献注录：Xiuping Zou • Erling Song • Aihong Peng •Yongrui He • Lanzhen Xu • Tiangang Lei •Lixiao Ya. Activation of three pathogen-inducible promoters in transgeniccitrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv.citri infection and wounding [J]. Plant Cell Tiss Organ Cult. 2014, 01: 85–98.
报/刊名：《Plant Cell Tiss Organ Cult》，发表于2014 / 第 01 期。
文献类型： [J]
页码：第 85–98 页 / 共14 页