作者：Chunzhen Cheng,Jiawei Yang,Hubin Yan,Xuejun Bei,Yongyan Zhang,Zhiming Lu,Guangyan Zhong
作者单位：College of Horticulture and landscape architecture, Southwest University, Chongqing 400715, P.R.China, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China, Economic Crops Station, Agricultural Bur
摘要：The Citrus tristeza virus (CTV) uses 3 silencing suppressor genes, p20, p23 and p25, to resist the attacks from its
Citrus hosts. Inactivating these genes is therefore obviously a potential defensive option in addition to the current
control strategies including aphid management and the use of mild strain cross protection. In this study, we cloned
partial DNA fragments from the three genes, and used them to construct vectors for expressing hairpin RNAs (hpRNAs).
To facilitate the formation of hpRNAs, the constructs were introduced a loop structure. Following transformation of
sour orange (Citrus aurantium) with these constructs, 8 p20 hpRNA (hp20) and 1 p25 hpRNA (hp25) expressing lines
were obtained. The 7 hp20 transgenic lines were further characterized. Their reactions to CTV were tested following
inoculation with CT14A and/or TR-L514, both of which are severe strains. Results showed that 3 lines (hp20-5, hp20-6
and hp20-8) were completely resistant to TR-L514 under greenhouse conditions for no detectable viral load was found
in their leaves by PCR. However, they exhibited only partial suppression of TR-L514 under screen house conditions
since the virus was detected in their leaves, though 2 months later compared to non-transgenic controls. Further tests
showed that hp20-5 was tolerant also to CT14A under screen house conditions. The growth of hp20-5 was much better
than others including the controls that were concurrently challenged with CT14A. These results showed that expressing p20 hpRNA was sufficient to confer sour orange with CTV resistance/tolerance.
关键词：Citrus tristeza virus (CTV), RNA interference, transgenic plant, Citrus aurantiu
文献注录：Chunzhen Cheng,Jiawei Yang,Hubin Yan,Xuejun Bei,Yongyan Zhang,Zhiming Lu,Guangyan Zhong. Expressing p20 hairpin RNA of Citrus tristeza virus confers Citrus aurantium with tolerance/resistance against stem pitting and seedling yellow CTV strains [J]. Journal of Integrative Agriculture. 2015.
报/刊名：《Journal of Integrative Agriculture》，发表于2015
文献类型： [J]
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