作者：Dutt Manjul,Barthe Gary,Irey Michael,Grosser Jude
作者单位：Citrus Research and Education Center, University of Florida, Lake Alfred, Florida, United States of America, Southern Gardens Citrus, Clewiston, Florida, United States of America
摘要：Commercial sweet orange cultivars lack resistance to Huanglongbing (HLB), a serious phloem limited bacterial disease that is usually fatal. In order to develop sustained disease resistance to HLB, transgenic sweet orange cultivars \'Hamlin\' and \'Valencia\' expressing an Arabidopsis thaliana NPR1 gene under the control of a constitutive CaMV 35S promoter or a phloem specific Arabidopsis SUC2 (AtSUC2) promoter were produced. Overexpression of AtNPR1 resulted in trees with normal phenotypes that exhibited enhanced resistance to HLB. Phloem specific expression of NPR1 was equally effective for enhancing disease resistance. Transgenic trees exhibited reduced diseased severity and a few lines remained disease-free even after 36 months of planting in a high-disease pressure field site. Expression of the NPR1 gene induced expression of several native genes involved in the plant defense signaling pathways. The AtNPR1 gene being plant derived can serve as a component for the development of an all plant T-DNA derived consumer friendly GM tree.
文献注录：Dutt Manjul,Barthe Gary,Irey Michael,Grosser Jude. Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening) [J]. PloS one. 2015, 10(9).
报/刊名：《PloS one》，发表于2015 / 第 10(9) 期。
文献类型： [J]
页码：第 页 / 共17 页