作者:Jahromi A. A.,Dardan M.
摘要:The present study was conducted to evaluate the effective factors on prosperity percent of shoot proliferation of Washington navel orange in vitro culture by using young flashes having bud in completely randomized design. For this purpose were selected young shoots having bud with length of 1.5 cm as explants and following disinfection by solution of sodium hypo chloride 1% and washing by distilled water were cultured in MS medium with 30% sucrose and 7.5 gl -1 Agar supplemented with BA (0.5, 1, 1.5, 2, 2.5 and 3 mgl -1) and NAA (0, 0.5, 0.75, 1, 1.25 and 1.5 mgl -1) and were placed in conditions of 16 hrs light and 8 hrs dark for 4-6 weeks. In the end of the experiment, were measured shoot number, shoot length, shoot quality, leaf number and the dropped leaf number. Based on the obtained results, the highest number and quality of the produced shoot was relative to BA 2 mgl -1 + NAA 0.5 mgl -1. The greatest shoot length obtained from medium containing 1 mgl -1 BA+1.5 mgl -1 NAA. The highest leaf number and the dropped leaf were observed in BA 2 mgl -1+NAA 0.5 mgl -1 and BA 1.5 mgl -1+NAA 0.5 mgl -1.
文献注录:Jahromi A. A.,Dardan M.. Evaluation the effective factors on shoot proliferation of Washington navel orange in tissue culture medium [J]. Indian Journal of Fundamental and Applied Life Sciences. 2015, 05(1): 244-248.
报/刊名:《Indian Journal of Fundamental and Applied Life Sciences》,发表于2015 / 第 05(1) 期。
文献类型: [J]
页码:第 244-248 页 / 共5 页