作者：Endo, T. Fujii, H. Sugiyama, A. Nakano, M. Nakajima, N. Ikoma, Y. Omura, M. Shimada, T
作者单位：NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Bio-oriented Research Organization (NARO), Shizuoka 424-0292, Japan. Electronic address: tomoen@affrc.go.jp.
摘要：To explore the transcription factors associated with carotenoid metabolism in citrus fruit, one transcription factor (CubHLH1) was selected through microarray screening in Satsuma mandarin (Citrus unshiu Marc.) fruit, which was treated with exogenous ethylene or gibberellin (GA), accelerating or retarding carotenoid accumulation in peel, respectively. The amino acid sequence of CubHLH1 has homology to Arabidopsis activation-tagged bri1 suppressor 1 (ATBS1) interacting factor (AIF), which is functionally characterized as a negative regulator of the brassinolide (BR) signalling pathway. Yeast two-hybrid analysis revealed that protein for CubHLH1 could interact with Arabidopsis and tomato ATBS1. Overexpression of CubHLH1 caused a dwarf phenotype in transgenic tomato (Solanum lycopersicum L.), suggesting that CubHLH1 has a similar function to Arabidopsis AIF. In the transgenic tomato fruit at ripening stage, the lycopene content was reduced along with the changes in carotenoid biosynthetic gene expression. The abscisic acid (ABA) content of all the transgenic tomato fruit was higher than that of the wild type. These results implied that CubHLH1 is considered to have a similar function to Arabidopsis AIFs and might be directly involved in carotenoid metabolism in mature citrus fruit.
关键词：Citrus, Brassinolide, bHLH, Transcription factor, Carotenoid, Light and arabidop
文献注录：Endo, T. Fujii, H. Sugiyama, A. Nakano, M. Nakajima, N. Ikoma, Y. Omura, M. Shimada, T. Overexpression of a citrus basic helix-loop-helix transcription factor (CubHLH1), which is homologous to Arabidopsis activation-tagged bri1 suppressor 1 interacting factor genes, modulates carotenoid [J]. Plant science : an international journal of experimental plant biology. 2015, 243: 35-48.
报/刊名：《Plant science : an international journal of experimental plant biology》，发表于2015 / 第 243 期。
文献类型： [J]
页码：第 35-48 页 / 共14 页