作者：Orbovic, V., Grosser, J. W.
摘要：The most frequently used method for production of citrus transgenic plants is via Agrobacterium -mediated transformation of tissues found on explants obtained from juvenile seedlings. Within the last decade and especially within the last 5–6 years, this robust method was employed to produce thousands of transgenic plants. With the newly applied screening methods that allow easier and faster detection of transgenic shoots, estimates of transformation rate for some cultivars have gone up making this approach even more attractive. Although adjustments have to be made regarding the (varietal) source of the starting material and Agrobacterium strain used in each experiment preformed, the major steps of this procedure have not changed signifi cantly if at all. Transgenic citrus plants produced this way belong to cultivars of rootstocks,sweet oranges, grapefruits, mandarins, limes, and lemons.
关键词：Agrobacterium tumefaciens , Citrus , Genetic transformation , Juvenile tissue
文献注录：Orbovic, V., Grosser, J. W.. Citrus transformation using juvenile tissue explants [J]. Agrobacterium Protocols，Methods Mol Biol. 2015, 2 (3): 245-257.
报/刊名：《Agrobacterium Protocols，Methods Mol Biol》，发表于2015 / 第 2 (3) 期。
文献类型： [J]
页码：第 245-257 页 / 共13 页