作者：Orbovic, V., Shankar, A., Peeples, M.E., Hubbard, C., and Zale, J.
作者单位：Citrus Research and Education Center, University of Florida/IFAS, 700 Experiment Station Road, Room 103, Lake Alfred, FL, 33850, USA, orbovic@ufl.edu.
摘要：Mature tissue protocol for production of transgenic Citrus plants via Agrobacterium-mediated transformation uses explants derived from branches of mature, fruit-bearing trees. Through the multiple cleaning steps consisting of grafting of apical tip meristems on rootstock plants grown under sanitary conditions, \"mother\" plants are produced that will serve as a source of budding material. These buds are grafted onto rootstock plants grown under the same, highly sanitary conditions. Newly obtained, one meter tall, young grafted plants serve as a source of explants for co-incubation experiments with Agrobacterium. Following successful transformation with Agrobacterium, selected transgenic shoots are micrografted onto rootstock plants in vitro where they are allowed to grow for a couple of months. Grafted transgenic plantlet together with the associated rootstock plant is taken out of culture tubes, severed from the root, and regrafted in terra on a 1-year-old rootstock plant. With the application of proper horticultural techniques, such a plant will yield first fruit about 12-15 months later.
关键词：Agrobacterium tumefaciens , Citrus , Genetic transformation , Mature tissue
文献注录：Orbovic, V., Shankar, A., Peeples, M.E., Hubbard, C., and Zale, J.. Citrus transformation using mature tissue explants [J]. Agrobacterium Protocols， Methods in Molecular Biology. 2015, Agrobacterium Protoc: 259-273.
报/刊名：《Agrobacterium Protocols， Methods in Molecular Biology》，发表于2015 / 第 Agrobacterium Protoc 期。
文献类型： [J]
页码：第 259-273 页 / 共15 页