作者：Xiaoqing Gong, Jingyan Zhang, Ji-Hong Liu
作者单位：Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China,E-mail address: liujihong@mail.hzau.edu.cn (J.-H. Liu).
摘要：Exploration of genes functioning in salt tolerance is crucial for generating transgenic plants with enhanced salt tolerance. In this study, we report the isolation and functional characterization of a stress-responsive gene FcSISP from Meiwa kumquat (Fortunella crassifolia). FcSISP encodes a putative protein of 47 amino acids, with a calculated molecular mass of 4.94 kDa and theoretical isoelectric point of 3.76, and was localized in the nucleus. Transcript levels of FcSISP were induced by dehydration, cold, salt and bacterium causing citrus canker, and hormones (salicylic acid and abscisic acid), with the greatest induction under salt treatment. Overexpression of FcSISP in tobacco (Nicotiana nudicaulis) conferred enhanced salt tolerance. The transgenic lines accumulated lower Na(+) contents, leading to reduced Na/K ratio, but accumulated more proline than the wild type (WT). Steady state mRNA levels of genes involved in Na(+) exchange (three SOS genes and three NHX genes) and proline synthesis (P5CS and P5CR) were higher in the transgenic lines in comparison with WT. Moreover, overexpression of FcSISP in trifoliate orange [Poncirus trifoliata (L.) Raf.], a widely-used and salt-sensitive citrus rootstock, led to elevated salt tolerance. Taken together, the data demonstrate that FcSISP plays a positive role in salt tolerance and that it holds a great potential for engineering salt tolerance in crops.
关键词：Salt stress tolerance,Fortunella crassifolia,Na exchange,Osmotic regulation,Ponc
文献注录：Xiaoqing Gong, Jingyan Zhang, Ji-Hong Liu. A stress responsive gene of Fortunella crassifolia FcSISP functions in salt stress resistance [J]. Plant Physiology and Biochemistry. 2014, 83: 10-19.
报/刊名：《Plant Physiology and Biochemistry》，发表于2014 / 第 83 期。
文献类型： [J]
页码：第 10-19 页 / 共10 页