作者： Sumontip Bunnag, Duangkamol Tangpong
作者单位：（Sumontip Bunnag）：Khon Kaen University, Applied Taxonomic Research Center, Department of Biology, Thailand, Muang Khon Kaen, Friendship Highway, 123/2001, 40002, e-mail: sumbun@kku.ac.th，（Duangkamol Tangpong）：Khon Kaen University, Department of Biolo
摘要：This study was carried out to optimize the conditions for plantlet regeneration and genetic
transformation of Citrus reticulata Blanco. with an antisense ACC oxidase gene mediated by
Agrobacterium tumefaciens. The highest percentage of plantlet regeneration of 100 was successfully
achieved on the MS medium supplemented with 3 μM BA, while the half-strength MS medium amended with 8 μM NAA was suitable for root induction. Explants that had high potency of regeneration were shoots, nodes and internodes, respectively. The delivery of an antisense ACC oxidase gene into the species C. reticulata mediated by Agrobacterium tumefaciens strain EHA 105 was successful by co-cultivating explants with the strain EHA105 for 15 min, following that by eliminating the bacterium with 200 mg/l cefotaxime, and subsequently selecting transformed embryoid with 20 mg/l hygromycin.Verified histochemically by GUS assay, putative transformants showed the percentage of gus gene expression of 100. Molecular analysis using PCR confirmed the integration of the antisense ACC oxidase gene into plant genome.
关键词：Citrus reticulata, Agrobacterium tumefaciens, antisense ACC oxidase, cefotaxime,
文献注录： Sumontip Bunnag, Duangkamol Tangpong. Delivery of an antisense ACC oxidase gene into Citrus reticulata Blanco. mediated by Agrobacterium tumefaciens. [J]. AAB Bioflux . 2013, 5(1): 29-38.
报/刊名：《AAB Bioflux 》，发表于2013 / 第 5(1) 期。
文献类型： [J]
页码：第 29-38 页 / 共10 页