作者：Wei, H. U.,Li, Yang,Xie, Yu Ming,Zhou, Xiao,Hong-Juan, G. E.,Da-Zhi, L. I.,Deng, Zi Niu
作者单位：湖南农业大学园艺园林学院，湖南 长沙 410128，国家柑橘改良中心长沙分中心，湖南 长沙 410128，Email:deng7009@163.com
摘要：To increase the genetic transformation efficiency of sweet orange infected by Agrobacterium,an explant derived from internode stem segments of sucarri orange seedling was adopted to optimize light regimes and co-cultivation condition for the growth.Meantime,seedless gene was transferred to succari orange.The results revealed that explant from seedling had higher regeneration and transformation rate under the condition of cultured 20 d in darkness first and then exposed 10 d in lighting environment.Acetosyringone(AS) concentration was a key factor impacting transformation rate,and the optimal performance of transformation was under 20 mg/L concentration of co-cultivated medium with a temperature 19 ℃ and pH 5.4–5.7.The positive rate could reach 29.4% through the analysis of GUS staining,which were further proved by PCR analysis that the foreign gene was indeed integrated into the host genome.
关键词：Citrus.sinennsis Osbeck cv.Succari , Agrobacterum-mediated , genetic transformat
文献注录：Wei, H. U.,Li, Yang,Xie, Yu Ming,Zhou, Xiao,Hong-Juan, G. E.,Da-Zhi, L. I.,Deng, Zi Niu. Further study on improving genetic transformation efficiency of succari orange [J]. Journal of Hunan Agricultural University . 2013, 39(4): 371-376.
基金: 现代农业(柑橘)产业技术体系岗位科学家项目(CAR–27)；湖南省重点项目(2011WK2007)；国家自然科学青年基金项目 (31201596)
报/刊名：《Journal of Hunan Agricultural University 》，发表于2013 / 第 39(4) 期。
文献类型： [J]
页码：第 371-376 页 / 共6 页