作者：Wang CX, Hong N, Wang GP, Jiang B, Fan XD
作者单位： The Key Laboratory of Plant Pathology of Hubei Province, National Indoor Conservation Center for Virus-free Gemplasm of Fruit Crop, College of Plant Science and Technology, Huazhong Agricultural University, Ministry of Education, Wuhan, Hubei 430070
摘要：Citrus tristeza virus isolate YC (CTV-YC), a stem pitting-inducing strain, was graft-transmitted to seedlings of Mexican lime [Citrus aurantifolia (Christm.)Swingle], Pineapple sweet orange [C. sinensis (L.) Osbeck] and Arizona Etrog citron 861-SI (C. medica L.).Nodal stem segments from CTV-YC-infected and healthy seedlings of the three species were used as explants for regeneration in vitro. CTV presence reduced significantly the proliferation rate, the vigour and the rooting capacity of regenerated shoots. The most severe effect was observed on Etrog citron, whose regeneration
ratio, shoot proliferation and root formation were inhibited to a great extent. CTV infection affected also the survival of transplanted plantlets. In vitro shoot cultures from CTV-infected explants of sweet orange and Mexican lime were successfully sub-cultured for up to 20-24 months, whereas those from infected Etrog usually survived less than 7 months. CTV could be detected in all sub-cultures, but its titer decreased with time.
关键词：Citrus tristeza virus, in vitro culture, morphogenesis, nodal segment, IC-RT-PCR
文献注录：Wang CX, Hong N, Wang GP, Jiang B, Fan XD. Effects of Citrus tristeza virus on teh growth of in vitro-cultured citrus [J]. Journal of Plant Pathology . 2009, 91 (2): 357-363.
报/刊名：《Journal of Plant Pathology 》，发表于2009 / 第 91 (2) 期。
文献类型： [J]
页码：第 357-363 页 / 共7 页