作者：Rafael do Prado Apparecido1 ， Eduardo Fermino Carlos2 ， Luciano Morais Lião3 ， Luiz Gonzaga Esteves Vieira4 ， Glaucia Braz Alcantara1
作者单位：Instituto de Química, Universidade Federal de Mato Grosso do Sul, Av. Filinto Müller, 1555, Campo Grande, MS 79074-460, Brazil 2 Instituto Agronômico do Paraná (IAPAR), Laboratório de Biotecnologia Vegetal, CP 481, Londrina, PR 86001-970, Br
摘要：Introduction Citrus canker, a disease caused by Xan-thomonas axonopodis pv. citri (Xac) bacteria, has been responsible for extensive economic losses in citriculture.In this work, we report the metabolic responses of citrus plants during disease development. This information can be useful for understanding the natural mechanism of plantdefense beyond helping design new varieties and/or geneti-cally modified genotypes for tolerance/resistance against citrus canker. Objectives To understand how primary metabolism is affected in two sweet orange genotypes during citrus can-
ker development.Methods 1H NMR spectroscopy together with chemo-metrics was used to evaluate the metabolic changes caused by Xac infection at various time points (days 4, 12 and 20)
in Citrus sinensis L. Osbeck leaves from non-transgenicand transgenic plants expressing the antibacterial peptide sarcotoxin.Results The results revealed a high level of metabolic similarity between the studied genotypes without Xac infec-tion. However, after Xac infection, the plants responded dif-ferently to disease development. The non-transgenic geno-type showed altered early precursors of some secondary metabolites (tryptophan, tyrosine and putrescine) in addi-tion to signaling metabolites of biotic stress (putrescine anddimethylamine), and the drastic reduction of gluconeogen-esis was the overall metabolic cost for defense. The trans-genic genotype suffered late metabolic changes due to theprotective stoichiometric role of sarcotoxin. In addition, the oxidative stress response was more balanced in transgenicthan in non-transgenic plants.Conclusion An NMR-based metabolomic approach was useful for understanding plant–pathogen interactions in citrus canker. Our findings provide valuable preliminary insights into different stages of citrus canker development.
关键词：NMR , Metabolic profiling , Xanthomonas axonopodis , Citrus sinensis , Plant–pat
文献注录：Rafael do Prado Apparecido1 ， Eduardo Fermino Carlos2 ， Luciano Morais Lião3 ， Luiz Gonzaga Esteves Vieira4 ， Glaucia Braz Alcantara1. NMR-based metabolomics of transgenic and non-transgenic sweet orange reveals different responses in primary metabolism during citrus canker development [J]. Metabolomics. 2017, 13.
报/刊名：《Metabolomics》，发表于2017 / 第 13 期。
文献类型： [J] （文献级别：SCI刊物）
页码：第 页 / 共12 页