作者：Sulley BenMahmoud a,John E. Ramos b, Robert G. Shatters Jr. b, David G. Hall b, Stephen L. Lapointe b, Randall P. Niedz b,PierreRougé c,Ronald D. Cave
作者单位：Indian River Research and Education Center, University of Florida, Fort Pierce, FL 34945, USA b United States Horticultural Research Laboratory, United States Department of Agriculture, Agricultural Research Service, 2001 South Rock Road, Ft. Pierce,
摘要：Diaprepes abbreviatus (L.) is an important pest of citrus inthe USA. Currently, no effectivemanagement strategies ofD. abbreviatus exist incitriculture, and newmethods of control are desperately sought. Toprotect citrus against D. abbreviatus a transgenic citrus rootstock expressing Bacillus thuringiensis Cyt2Ca1, an insect toxin protein,was developed using Agrobacterium-mediated transformation of ‘Carrizo’ citrange [Citrus sinensis (L) Osbeck Poncirus trifoliate (L) Raf]. The transgenic citrus root stock expressed the cytolytic toxin Cyt2Ca1 constitutively under the control of a 35S promoter in the transgenic Carrizo citrange trifoliate hybrid including the roots that are the food source of larval D. abbreviatus. The engineered citrus was screened by Western blot and RT-qPCR analyses for cyt2Ca1 and positive citrus identified. Citrus trees expressing different levels of cyt2Ca1 transcriptswere identi-
fied (Groups A–C). High expression of the toxin in the leaves (109 transcripts/ng RNA), however, retarded plant growth. The transgenic plantswere grown inpots and the roots exposed to 3week oldD. abbreviatus larvae using no-choice plant bioassays. Three cyt2Ca1 transgenic plants were identified that sustained less root damage be- longing to Group B and C. One plant caused death to 43% of the larvae that fed on its roots expressed 8 × 106 cyt2Ca1 transcripts/ng RNA. These results show, for the first time, that Cyt2Ca1 expressed in moderate amountsby the roots of citrus does not retard citrus growth and can protect it fromlarval D. abbreviatus.
关键词：Diaprepes abbreviatus Transgenic citrus rootstock cyt2Ca1 Larval control Root re
文献注录：Sulley BenMahmoud a,John E. Ramos b, Robert G. Shatters Jr. b, David G. Hall b, Stephen L. Lapointe b, Randall P. Niedz b,PierreRougé c,Ronald D. Cave. Expression of Bacillus thuringiensis cytolytic toxin (Cyt2Ca1) in citrus roots to control Diaprepes abbreviatus larvae [J]. Pesticide Biochemistry and Physiology. 2016, 136: 1-11.
报/刊名：《Pesticide Biochemistry and Physiology》，发表于2016 / 第 136 期。
文献类型： [J] （文献级别：SCI刊物）
页码：第 1-11 页 / 共11 页