作者：Zhang, Yong-yan Zhang, Dong-min Zhong, Yun Chang, Xiao-jun Hu, Min-lun Cheng, Chun-zhen
作者单位：Corresponding author at: College of Horticulture, FujianAgriculture and Forestry University, Cangshan, Fuzhou, 350002, China.
摘要：A simple and efficient transformation method is important for plant genetic breeding and gene function analysis. In this study, we developed a in planta transformation approach for pommelo (Citrus maxima), which includes seed germination, seedling decapitation, Parafilm funnel-type-winding, Agrobacterium infection, co-culture, selective-culture, dark treatment for buds induction, transgene integration confirmation and transgenic plant propagation. The highest transformation efficiency (20.41%) was obtained when using hygromycin as selection marker, followed by Basta (1937%) and Kanamycin (3.21%). Trans genic plants obtained using this method could be graft-propagated in 3 months post transformation, which is shorter than the tissue-culture-dependent transformation. The transformation protocol presented here has great potential to be used for citrus genetic breeding and improvement. (C) 2016 Elsevier B.V. All rights reserved.
关键词：Agrobacterium-mediated transformation Citrus maxima Dark incubation in planta tr
文献注录：Zhang, Yong-yan Zhang, Dong-min Zhong, Yun Chang, Xiao-jun Hu, Min-lun Cheng, Chun-zhen. A simple and efficient in planta transformation method for pommelo (Citrus maxima) using Agrobacterium tumefaciens [J]. Scientia Horticulturae. 2016, 214: 174-179.
报/刊名：《Scientia Horticulturae》，发表于2016 / 第 214 期。
文献类型： [J] （文献级别：SCI刊物）
页码：第 174-179 页 / 共6 页