作者：De Francesco, A. Costa, N. Garcia, M. L.
作者单位：Instituto de Biotecnologı′a y Biologı′a Molecular, CCT-La Plata, CONICET - UNLP, calles 47 y 115, 1900 La Plata, Buenos Aires, Argentina
摘要：Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.
关键词：Citrus psorosis virus ? Hairpin-RNA ? Psorosis A ? Psorosis B ? Transgenic sweet
文献注录：De Francesco, A. Costa, N. Garcia, M. L.. Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes [J]. Transgenic Research. 2016, 02: 225-235.
报/刊名：《Transgenic Research》，发表于2016 / 第 02 期。
文献类型： [J] （文献级别：SCI刊物）
页码：第 225-235 页 / 共11 页