作者：Grosser, J. W., Jr, Gmitter Fg, Tusa, N and Chandler, J. L.
摘要：Abstract Allotetraploid intergeneric somatic hybrid plants between Citrus reticulata Blanco cv. Cleopatra mandarin and Citropsis gilletiana Swing. & M. Kell. (common name Gillet\'s cherry orange) were regenerated following protoplast fusion. Cleopatra protoplasts were isolated from an ovule-derived embryogenic suspension culture and fused chemically with leaf-derived protoplasts of Citropsis gilletiana. Cleopatra mandarin and somatic hybrid plants were regenerated via somatic embryogenesis. Hybrid plant identification was based on differential leaf morphology, root-tip cell chromosome number, and electrophoretic analyses of phosphoglucose mutase (PGM) and phosphohexose isomerase (PHI) isozyme banding patterns. This is the first somatic hybrid within the Rutaceae reported that does not have Citrus sinensis (sweet orange) as a parent, and the first produced with a commercially important citrus rootstock and a complementary but sexually incompatible, related species.
关键词：embryogenesis, protoplast fusion, regeneration, Citrus reticulata, citropsis gil
文献注录：Grosser, J. W., Jr, Gmitter Fg, Tusa, N and Chandler, J. L.. Somatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana [J]. Plant Cell Reports. 199: 656-659.
报/刊名：《Plant Cell Reports》，发表于 199
文献类型： [J]
页码：第 656-659 页 / 共 页