作者：Long, Jian Mei, Liu, Chao Yang, Feng, Meng Qi, Liu, Yun, Wu, Xiao Meng and Guo, Wen Wu
摘要：Overexpression of csi-miR156a enhances the somatic embryogenesis capability of citrus through downstream repression ofCsSPL3andCsSPL14. miR156 is a highly conserved plant miRNA and has been extensively studied because of its versatile roles in plant development. Here, we report a novel role of miR156 in regulating somatic embryogenesis (SE) in citrus, one of the most widely cultivated fruit crops in the world. SE is an important means ofin vitroregeneration, but over the course of long-term sub-culturing there is always a decline in the SE potential of the preserved citrus embryogenic callus, and this represents a key obstacle for citrus biotechnology. In this study, the SE competence of citrus callus of wild kumquat (Fortunella hindsii) was significantly enhanced by either overexpression of csi-miR156a or by individual knock-down of the two target genes,CsSPL3andCsSPL14, indicating that the effect of miR156-SPLmodules was established during the initial phases of SE induction. Biological processes that might promote SE in response to miR156 overexpression were explored using RNA-seq, and mainly included hormone signaling pathways, stress responses, DNA methylation, and the cell cycle. CsAKIN10 was identified as interacting protein of CsSPL14. Our results provide insights into the regulatory pathway through which miR156-SPLmodules enhance the SE potential of citrus callus, and provide a theoretical basis for improvement of plant SE competence.
关键词： Callus citrus knock-down miR156 overexpression somatic embryogenesis SPL
文献注录：Long, Jian Mei, Liu, Chao Yang, Feng, Meng Qi, Liu, Yun, Wu, Xiao Meng and Guo, Wen Wu. miR156-SPLmodules regulate induction of somatic embryogenesis in citrus callus [J]. Journal of Experimental Botany. 2018: 2979-2993.
报/刊名：《Journal of Experimental Botany》，发表于2018
文献类型： [J]
页码：第 2979-2993 页 / 共 页