作者：Lin W, Zhang S, Ding F, He X, Luo C, Huang G, Do M, Wang Q, Yang Z, Su L, Hu G
摘要：Gametophytic self-incompatibility (GSI) is an important factor affecting fruit development and is one of the important mechanisms of seedlessness in citrus. Although many studies have investigated the mechanisms of SI, this process remains unclear in Xiangshui lemon (C. limon). In this study, we cloned one S-RNase homologous gene and one SLF homologous gene from the Xiangshui lemon, designated as ClS1 (pistil-specific expression) and ClF-box (pollen-specific expression), which contain open reading frames (ORFs) of 534 and 810bp, respectively. The expression pattern of these two genes was analyzed by quantitative real-time polymerase chain reaction, and the results showed that the expression pattern of these two genes was significantly upregulated after self-pollination compared to that after cross-pollination. Through pollination in transgenic tobacco, we found that the pollen tubes grew slowly and became twisted after cross-pollination of ClS1f and ClF-box o transgenic tobaccos, but the pollen tubes of control, self-pollination ClS1 and cross-pollination ClS1o, and ClF-box f grew normally. Furthermore, significantly fewer pollen tubes of ClS1f and ClF-box o entered the ovules than that in other combinations, and the seed number of cross-pollination ClS1f and ClF-box o transgenic tobaccos was significantly reduced. These results indicated that the ClS1 and ClF-box genes contribute to the self-incompatibility of the Xiangshui lemon.
文献注录：Lin W, Zhang S, Ding F, He X, Luo C, Huang G, Do M, Wang Q, Yang Z, Su L, Hu G. Two Genes (ClS1 and ClF-box) Involved the Self-Incompatibility of "Xiangshui" Lemon (Citrus limon (L.) Burm. f.) [J]. Plant Molecular Biology Reporter. 2019, 37: 50-62.
报/刊名：《 Plant Molecular Biology Reporter》，发表于2019 / 第 37 期。
文献类型： [J]
页码：第 50-62 页 / 共2 页