作者：Peng A, Zou X, Xu L, He Y, Lei T, Yao L, Li Q, Chen S
摘要：The production of transgenic citrus plants from adult tissues is difficult because of low regeneration and transformation rates. To increase the transformation efficiency of adult citrus tissues, an improved protocol involving adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues was developed. Explants were pre-incubated in a liquid medium prior to infection by Agrobacterium tumefaciens. Plant materials were also incubated on callus-induction medium supplemented with various combinations of cytokinin (Cyt) and kanamycin (Kan). An appropriate pre-incubation of the explants increased the transformation efficiency of adult tissues. During the callus-induction period, the Cyt type and Kan concentration had the largest and smallest effects on the transformation efficiency, respectively. The most effective combination of plant growth regulator and Kan for the transformation of ‘Tarocco’ blood orange tissues was 2 mg L⁻¹ 2-isopentenyl adenine and 50 mg L⁻¹ Kan. The transformation efficiency under the optimized conditions was 11.7%. A Southern blot analysis confirmed the integration of the transgene. These results indicated that the transformation efficiency of adult citrus tissues can be enhanced by optimizing the transformation conditions.
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文献注录：Peng A, Zou X, Xu L, He Y, Lei T, Yao L, Li Q, Chen S. Improved protocol for the transformation of adult Citrus sinensis Osbeck ‘Tarocco’ blood orange tissues [J]. In Vitro Cellular & Developmental Biology - Plant. 2019.
报/刊名：《In Vitro Cellular & Developmental Biology - Plant》，发表于2019
文献类型： [J]
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