作者：Sendin Lorena Noelia, Filippone Maria Paula
摘要：Developing disease resistance is one of the most important components of any plant breeding program. Citrus traditional breeding methods (bud sport selection, crossbreeding, and other breeding channels) are a laborious task and often hampered by long juvenility, a high degree of heterozygosity, polyembryony, self-incompatibility, and abortion of reproductive organs. An interesting alternative to the classical breeding approach is the use of genetic transformation, which provides the means for adding a single agronomic trait to a plant without otherwise altering its phenotype. Agrobacterium tumefaciens-mediated transformation has been carried out with numerous hybrids and citrus species. This technique allowed us to introduce the Bs2 gene in Citrus, as well as to increase citrus canker resistance in transgenic Bs2 gene-expressing lines.
文献注录：Sendin Lorena Noelia, Filippone Maria Paula. The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker [J]. Methods in molecular biology (Clifton, N.J.). 2019: 179-190.
报/刊名：《Methods in molecular biology (Clifton, N.J.)》，发表于2019
文献类型： [J]
页码：第 179-190 页 / 共11 页