标题:Auto- and mutual-regulation between two CitERFs contribute to ethylene-induced citrus fruit degreening

作者:Li Shao-Jia, Xie Xiu-Lan, Liu Sheng-Chao, Chen Kun-Song, Yin Xue-Ren

作者单位:<Go to ISI>://MEDLINE:31319344

摘要:Citrus fruit postharvest degreening is a critical stage in marketing, carried out by exposure to ethylene or ethephon. Genome-wide screening of the AP2/ERF superfamily indicated that a novel ERF-II (CitERF6) was shown to trans-activate the CitPPH promoter. Expression of CitERF6 is associated with both developmental and postharvest degreening in citrus fruit. Transient and stable over-expression of CitERF6 in Nicotiana tabacum leaves and 'Ponkan' fruit also results in rapid chlorophyll degradation. Auto- and mutual-regulation was also found between CitERF6 and the previously characterized CitERF13 using the dual-luciferase and yeast one-hybrid assays. Moreover, substitution of the 35S promoter for endogenous promoters showed that both pCitERF6::CitERF6 and pCitERF13::CitERF13 were effective in trans-activating their promoters or triggering chlorophyll degradation. It is proposed that ethylene is one of the triggers activating promoters of CitERF6 and CitERF13, and subsequent auto- and mutual-regulation between CitERF6 and CitERF13 might facilitate the effect of ethylene, leading to fruit degreening.
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文献注录:Li Shao-Jia, Xie Xiu-Lan, Liu Sheng-Chao, Chen Kun-Song, Yin Xue-Ren. Auto- and mutual-regulation between two CitERFs contribute to ethylene-induced citrus fruit degreening [J]. Food chemistry. 2019: 125163-125163.

报/刊名: Food chemistry》,发表于2019

文献类型: [J]

页码: 125163-125163 页 / 共1

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