作者：Zhang, Shuai; Yin, Zhao-Ping; Wu, Xiao-Meng; Li, Chao-Chao; Xie, Kai-Dong; Deng, Xiu-Xin; Grosser, Jude W.; Guo, Wen-Wu
摘要：Cytoplasmic male sterility (CMS) and seedlessness are highly desirable traits for citrus. However, a high-quality reference mitochondrial genome of citrus is unavailable, and the mechanisms of CMS remain to be elucidated in citrus. We previously generated a male sterile somatic cybrid of pummelo (G1+HBP) by fusing protoplasts from CMS 'Guoqing No. 1' Satsuma mandarin (G1) and fertile 'Hirado Buntan' pummelo (HBP). Here, we sequenced and assembled the mitochondrial genome of G1, G1+HBP, and HBP into circular molecules of 521.6 kb, 538.4 kb, and 518.3 kb, respectively. Mitochondrial genome of citrus encodes 35-36 proteins, three ribosomal RNAs, and 21 tRNAs. Comparative analysis indicated that nuclear genome and chloroplast genome of the cybrid are from HBP, whereas the mitochondrial genome sequences of the cybrid and G1 have 96.9% synteny, and that the cybrid has an extra recombinant mitochondrial DNA fragment of only 16.8 kb. Compared with the fertile HBP mitochondrial genome, 21 and 20 CMS-specific mitotype-specific sequences (MSSs), and 89 and 70 CMS-specific ORFs were identified in the cybrid and G1 mitochondrial genomes, respectively. Among them, 57 ORFs that co-transcribed with the same mitochondrial genes in the cybrid and G1 were identified as the CMS-specific ORFs. The assembly of mitochondrial genomes and the identification of the CMS-specific ORFs provide basis for determination of the CMS-associated gene and elucidation of CMS mechanisms in citrus.
文献注录：Zhang, Shuai; Yin, Zhao-Ping; Wu, Xiao-Meng; Li, Chao-Chao; Xie, Kai-Dong; Deng, Xiu-Xin; Grosser, Jude W.; Guo, Wen-Wu. Assembly of Satsuma mandarin mitochondrial genome and identification of cytoplasmic male sterility-specific ORFs in a somatic cybrid of pummelo [J]. . 2020.
报/刊名：《》，发表于2020
文献类型： [J]
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