标题:DNA fingerprinting: A novel technique for identification of important species of citrus

作者:Tupke, A. H.; Akhare, A. A.; Gahukar, S. J.; Bahatkar, B. P.; Zadokar, A. R.; Gaikwad, P. N.; Katkade, R. R.

摘要:In the present investigation, seven different species specific SCAR markers were used for discriminating Galgal rootstock from other citrus species under study. The PDKV-1, PDKV-2, PDKV-3, PDKV-4, PDKV-5, PDKV-6 and PDKV-7 show the band size of 283 bp, 159 bp, 172 bp, 347 bp, 150 bp, 137 bp and 347 bp, respectively. The PIC value ranged from 0.38 to 0.88. The highest PIC value was observed in PDKV-7 (0.88), followed by PDKV-1 (0.86) and PDKV-2 (0.83). Out of these primers, PDKV-1, PDKV-2, PDKV-6 and PDKV-7 are the potential markers for Galgal discrimination from Rangpur lime, Alemow, Jambhiri, Orange and Sweet orange. Molecular characterization of citrus species revealed highest similarity between Jambhiri and Alemow while the lowest similarity was observed between Alemow and Galgal. The cluster analysis revealed that Galgal rootstock is more diverse from other five species under study. These results will be very useful in testing the genetic purity of citrus at nursery stage.

文献注录:Tupke, A. H.; Akhare, A. A.; Gahukar, S. J.; Bahatkar, B. P.; Zadokar, A. R.; Gaikwad, P. N.; Katkade, R. R.. DNA fingerprinting: A novel technique for identification of important species of citrus [J]. . 2021: 202-207.

报/刊名:》,发表于2021

文献类型: [J]

页码: 202-207 页 / 共

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