作者：Zhang, Yingzi, Jin, Jiajing, Zhu, Shenchao, Sun, Quan, Zhang, Yin, Xie, Zongzhou, Ye, Junli and Deng, Xiuxin
摘要： As an essential horticultural crop, Citrus has carotenoid diversity, which affects its aesthetic and nutritional values. beta,beta-Xanthophylls are the primary carotenoids accumulated in citrus fruits, and non-heme di-iron carotene hydroxylase (BCH) enzymes are mainly responsible for beta,beta-xanthophyll synthesis. Previous studies have focused on the hydroxylation of BCH1, but the role of its paralogous gene in citrus, BCH2, remains largely unknown. In this study, we revealed the beta-hydroxylation activity of citrus BCH2 (CsBCH2) for the first time through the functional complementation assay using Escherichia coli, although CsBCH2 exhibited a lower activity in hydroxylating beta-carotene into beta-cryptoxanthin than citrus BCH1 (CsBCH1). Our results showed that overexpression of CsBCH2 in citrus callus increased xanthophyll proportion and plastoglobule size with feedback regulation of carotenogenic gene expression. This study revealed the distinct expression patterns and functional characteristics of two paralogous genes, CsBCH1 and CsBCH2, and illustrated the backup compensatory role of CsBCH2 for CsBCH1 in citrus xanthophyll biosynthesis. The independent function of CsBCH2 and its cooperative function with CsBCH1 in beta-cryptoxanthin biosynthesis suggested the potential of CsBCH2 to be employed for expanding the synthetic biology toolkit in carotenoid engineering.
文献注录：Zhang, Yingzi, Jin, Jiajing, Zhu, Shenchao, Sun, Quan, Zhang, Yin, Xie, Zongzhou, Ye, Junli and Deng, Xiuxin. Citrus beta-carotene hydroxylase 2 (BCH2) participates in xanthophyll synthesis by catalyzing the hydroxylation of beta-carotene and compensates for BCH1 in citrus carotenoid metabolism [J]. . 2023.
报/刊名：《》，发表于2023
文献类型： [J]
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