作者：Yue, Pengtao, Jiang, Zhenghua, Sun, Quan, Wei, Ranran, Yin, Yingzi, Xie, Zongzhou, Larkin, Robert M., Ye, Junli, Chai, Lijun and Deng, Xiuxin
摘要：Carotenoids are natural pigments that influence the color of citrus fruit. The red-colored carotenoid beta-citraurin is responsible for the peel color in "Newhall" orange (Citrus sinensis). Although jasmonates are known to regulate the biosynthesis and accumulation of carotenoids, their effects on beta-citraurin biosynthesis in citrus fruit remain unclear. Here, we determined that treatment with methyl jasmonate (MeJA) significantly promotes fruit coloration and beta-citraurin production in "Newhall" orange. A MeJA treatment induced the expression of CsMYC2, which encodes a transcription factor that serves as a master regulator of jasmonate responses. CsMYC2 bound the promoter of the gene that encodes carotenoid cleavage dioxygenase 4b (CsCCD4b), the key gene for beta-citraurin biosynthesis, and the promoters of genes that encode phytoene synthase (CsPSY), lycopene beta-cyclase (CsLCYb), and beta-carotene hydroxylase (CsBCH) and induced their expression. In addition, CsMYC2 promoted CsMPK6 expression. Notably, we found that CsMPK6 interacted with CsMYC2 and that this interaction decreased the stability and DNA-binding activity of CsMYC2. Thus, we conclude that negative feedback regulation attenuates JA signaling during the jasmonate-induced coloration of citrus fruit. Together, our findings indicate that jasmonates induce beta-citraurin biosynthesis in citrus by activating a CsMPK6-CsMYC2 cascade, thereby affecting fruit coloration.
文献注录：Yue, Pengtao, Jiang, Zhenghua, Sun, Quan, Wei, Ranran, Yin, Yingzi, Xie, Zongzhou, Larkin, Robert M., Ye, Junli, Chai, Lijun and Deng, Xiuxin. Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of beta-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis) [J]. . 2023.
报/刊名：《》，发表于2023
文献类型： [J]
页码：第 页 / 共 页