作者：Mahmoud, Lamiaa M., Kaur, Prabhjot, Stanton, Daniel, Grosser, Jude W. and Dutt, Manjul
摘要： Background The genetic engineering of crops has enhanced productivity in the face of climate change and a growing global population by conferring desirable genetic traits, including the enhancement of biotic and abiotic stress tolerance, to improve agriculture. The clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system has been found to be a promising technology for genomic editing. Protoplasts are often utilized for the development of genetically modified plants through in vitro integration of a recombinant DNA fragment into the plant genome. We targeted the citrus Nonexpressor of Pathogenesis-Related 3 (CsNPR3) gene, a negative regulator of systemic acquired resistance (SAR) that governs the proteasome-mediated degradation of NPR1 and developed a genome editing technique targeting citrus protoplast DNA to produce stable genome-edited citrus plants. Results Here, we determined the best cationic lipid nanoparticles to deliver donor DNA and described a protocol using Lipofectamine (TM) LTX Reagent with PLUS Reagent to mediate DNA delivery into citrus protoplasts. A Cas9 construct containing a gRNA targeting the CsNPR3 gene was transfected i
文献注录：Mahmoud, Lamiaa M., Kaur, Prabhjot, Stanton, Daniel, Grosser, Jude W. and Dutt, Manjul. A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants [J]. . 2022, 1.
报/刊名：《》，发表于2022 / 第 1 期。
文献类型： [J]
页码：第 页 / 共 页