作者：Catalano, Chiara, Casas, Giuseppina Las, Giuffrida, Alessio, Ferlito, Filippo, Di Guardo, Mario, Continella, Alberto, Bennici, Stefania, La Malfa, Ste
摘要：Background: Floral sterility and nucellar embryony are peculiar traits of several Citrus species and represent an obstacle to traditional breeding. Morphological sterility mainly results in pistil abortion and anther atrophy, while polyembryony is due to a mechanism known as sporophytic apomixis, which consists of the presence of embryos in the seed generated from the nucellar (maternal) tissue alongside the zygotic embryo (sexual origin). Considering the growing interest in lemon [Citrus limon (L.) Burm. f.] breeding, and the lack of information on floral sterility and the polyembryony trait among different lemon cultivars, a morphological and molecular characterization of these traits of interest was performed on forty Sicilian and international lemon cultivars available in the citrus germplasm collection of Catania University (Italy). Methods: Eight traits related to the reproductive biology were assessed on the selected lemon cultivars, namely: pistil abortion and anther atrophy, number of seeds per fruit, number of embryos per seed, percentage of seeds showing polyembryony, germination, percentage of seeds resulting in more than one plantlet, and average seed weight. Moreover, seedlings recovered after the germination assay were genotyped with SNP and SSR markers for ascertaining their nucellar or zygotic origin. In addition, PCR analysis were performed to assess the allele combination of the miniature inverted-repeat transposable element (MITE) insertion in CitRKD1, a gene associated with the occurrence of apomixis in citrus. Results: All traits showed high variability among the accessions analyzed. As for polyembryony, lemon 'Adamopoulos' scored the highest percentage of polyembryonic seeds (67.6%), whilst lemon 'Lunario' showed the lowest value (8.7%). Conclusions: Insights on the level of polyembryony within lemon varieties will represent a valuable tool for breeders for the set-up of novel mating schemes. In fact, when a polyembryonic female parent is used in cross breeding, the selection of the zygotic individual is hampered by the presence of a nucellar one.
文献注录：Catalano, Chiara, Casas, Giuseppina Las, Giuffrida, Alessio, Ferlito, Filippo, Di Guardo, Mario, Continella, Alberto, Bennici, Stefania, La Malfa, Ste. Reproductive Biology Factors Hampering Lemon Citrus limon (L.) Burm. f. Genetic Improvement [J]. . 2022, 12.
报/刊名：《》，发表于2022 / 第 12 期。
文献类型： [J]
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