作者：Fang, Hong, Culver, James N., Niedz, Randall P. and Qi, Yiping
摘要：Clustered regularly interspaced short palindromic repeats (CRISPR) technology is a powerful genome editing tool. Recently developed CRISPR-Cas12a system confers several advantages over CRISPR-Cas9, making it ideal for use in plant genome editing and crop improvement. While traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and off-target effects, CRISPR-Cas12a delivered as ribonucleoproteins (RNPs) can effectively alleviate these potential Issues. Here we present a detailed protocol for LbCas12a-mediated genome editing using RNP delivery in Citrus protoplasts. This protocol provides a comprehensive guideline for RNP component preparation, RNP complex assembly and delivery, and editing efficiency assessment.
文献注录：Fang, Hong, Culver, James N., Niedz, Randall P. and Qi, Yiping. Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line [J]. . 2023: 153-171.
报/刊名：《》，发表于2023
文献类型： [J]
页码：第 153-171 页 / 共 页