作者：Hu Jianbing, Liu Chenchen, Du Zezhen,Guo Furong,Song Dan,Wang Nan,Wei Zhuangmin,Jiang Jingdong,Cao Zonghong,Shi Chunmei,Zhang Siqi,Zhu Chenqiao,Chen P
摘要：Self-incompatibility (SI) is a widespread prezygotic mechanism for flowering plants to avoid inbreeding depression and promote genetic diversity. Citrus has an S-RNase-based SI system, which was frequently lost during evolution. We previously identified a single nucleotide mutation in Sm -RNase, which is responsible for the loss of SI in mandarin and its hybrids. However, little is known about other mechanisms responsible for conversion of SI to self-compatibility (SC) and we identify a completely different mechanism widely utilized by citrus. Here, we found a 786-bp miniature inverted-repeat transposable element (MITE) insertion in the promoter region of the FhiS2 -RNase in Fortunella hindsii Swingle (a model plant for citrus gene function), which does not contain the Sm -RNase allele but are still SC. We demonstrate that this MITE plays a pivotal role in the loss of SI in citrus, providing evidence that this MITE insertion prevents expression of the S-RNase; moreover, transgenic experiments show that deletion of this 786-bp MITE insertion recovers the expression of FhiS2 -RNase and restores SI. This study identifies the first evidence for a role for MITEs at the S-locus affecting the SI phenotype. A family-wide survey of the S-locus revealed that MITE insertions occur frequently adjacent to S-RNase alleles in different citrus genera, but only certain MITEs appear to be responsible for the loss of SI. Our study provides evidence that insertion of MITEs into a promoter region can alter a breeding strategy and suggests that this phenomenon may be broadly responsible for SC in species with the S-RNase system.
文献注录：Hu Jianbing, Liu Chenchen, Du Zezhen,Guo Furong,Song Dan,Wang Nan,Wei Zhuangmin,Jiang Jingdong,Cao Zonghong,Shi Chunmei,Zhang Siqi,Zhu Chenqiao,Chen P. Transposable elements cause the loss of self-incompatibility in citrus [J]. . 2023.
报/刊名：《》，发表于2023
文献类型： [J]
页码：第 页 / 共 页