作者：Huang, Xiaoen, Jia, Hongge, Xu, Jin, Wang, Yuanchun, Wen, Jiawen and Wang, Nian
摘要：Transgene-free plant genome editing in the T0 generation is highly desirable but challenging1,2. Here we achieved such a goal using a co-editing strategy via Agrobacterium-mediated transient expression of cytosine base editor to edit ALS encoding acetolactate synthase to confer herbicide chlorsulfuron resistance as a selection marker, Cas12a/CRISPR RNA for editing gene(s) of interest, and green fluorescent protein for selecting transgene-free transformants. The biallelic/homozygous transgene-free mutation rates for target genes among herbicide-resistant transformants ranged from 1.9% to 42.1% in tomato, tobacco, potato and citrus. This co-editing strategy is particularly useful for transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation. Huang et al. develop a potent genome editing toolkit to generate transgene-free genome-edited plants in the T0 generation by co-editing of ALS gene (without obvious fitness costs) and gene(s) of interest via Agrobacterium-mediated transient expression.
文献注录：Huang, Xiaoen, Jia, Hongge, Xu, Jin, Wang, Yuanchun, Wen, Jiawen and Wang, Nian. Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy [J]. . 2023.
报/刊名：《》，发表于2023
文献类型： [J]
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